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The 2 protein bands from 40 kDa to 30 kDa have been absent soon after 24 h incubation at forty five °C. For the remainder of the bands, many concentrations of all a few proteases retained protein bands corresponding to their authentic molar masses (Figure 6a,b). The existence of bands at low molecular fat in fish roe ahead of hydrolysis was unanticipated. While in the report by Ghelichi et al. [8], minor peaks of a relatively low molecular pounds variety (3–fourteen kDa) have been observed in defatted carp roe homogenate, probably due to an autolytic procedure occurring in fish roe and/or maybe the defatting approach leading to the development of very low-molecular-bodyweight peptides.
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